Isolation and characterization of mutants of Bacillus thuringiensis var. israelensis |
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| Authors: | H.-S. Kim,J.-C. Cô té ,S. Fré chette,Y.S. Chung |
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| Affiliation: | Département de Sciences Biologiques, Universitéde Montréal, Montréal;Station de Recherches, Agriculture Canada, St-Jean-sur-Richelieu, Québec, Canada |
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| Abstract: | Several mutants of Bacillus thuringiensis var. israelensis HD-500 (Bti) were obtained after treatment with N -methyl-n'-nitro- N -nitrosoguanidine. On the basis of the production (+) or absence (-) of spore (Spo) and crystal (Cry) the strains were grouped into three categories: Spo + Cry +, Spo + Cry - and Spo - Cry + . NGI-22, a Spo + Cry - mutant lacked all crystal proteins. Both NGI-23 and NGI-23-1 were Spo - Cry + . NGI-23-1, however, produced multiple crystals per cell. These mutants could prove useful not only for analysing sporulation and crystal formation as well as the linkage between the two genetic processes but also for improving the potential of Bti as a microbial insecticide. |
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