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A Label-Free Luminescent Switch-On Assay for ATP Using a G-Quadruplex-Selective Iridium(III) Complex |
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| Authors: | Ka-Ho Leung Lihua Lu Modi Wang Tsun-Yin Mak Daniel Shiu-Hin Chan Fung-Kit Tang Chung-Hang Leung Hiu-Yee Kwan Zhiling Yu Dik-Lung Ma |
| Affiliation: | 1. Department of Chemistry, Hong Kong Baptist University, Kowloon Tong, Hong Kong, China.; 2. State Key Laboratory of Quality Research in Chinese Medicine, Institute of Chinese Medical Sciences, University of Macau, Macao, China.; 3. Center for Cancer and Inflammation Research, School of Chinese Medicine, Hong Kong Baptist University, Hong Kong, China.; Queen’s University Belfast, United Kingdom, |
| Abstract: | We report herein the G-quadruplex-selective property of a luminescent cyclometallated iridium(III) complex for the detection of adenosine-5′-triphosphate (ATP) in aqueous solution. The ATP-binding aptamer was employed as the ATP recognition unit, while the iridium(III) complex was used to monitor the formation of the G-quadruplex structure induced by ATP. The sensitivity and fold enhancement of the assay were higher than those of the previously reported assay using the organic dye crystal violet as a fluorescent probe. This label-free luminescent switch-on assay exhibits high sensitivity and selectivity towards ATP with a limit of detection of 2.5 µM. |
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