Recombination-Dependent Oligomerization of Human Papillomavirus Genomes upon Transient DNA Replication |
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| Authors: | Marit Orav Liisi Henno Helen Isok-Paas Jelizaveta Geimanen Mart Ustav Ene Ustav |
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| Institution: | University of Tartu, Institute of Technology, Tartu, Estoniaa;Estonian Biocentre, Tartu, Estoniab;Icosagen Cell Factory OÜ, Tartu, Estoniac;Estonian Academy of Sciences, Tallinn, Estoniad |
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| Abstract: | We describe the extensive and progressive oligomerization of human papillomavirus (HPV) genomes after transfection into the U2OS cell line. The HPV genomic oligomers are extrachromosomal concatemeric molecules containing the viral genome in a head-to-tail orientation. The process of oligomerization does not depend on the topology of the input DNA, and it does not require any other viral factors besides replication proteins E1 and E2. We provide evidence that oligomerization of the HPV18 and HPV11 genomes involves homologous recombination. We also demonstrate oligomerization of the HPV18 and HPV11 genomes in SiHa, HeLa, and C-33 A cell lines and provide examples of oligomeric HPV genomes in clinical samples obtained from HPV-infected patients. |
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