Resveratrol Protects Mouse Oocytes from Methylglyoxal-Induced Oxidative Damage |
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| Authors: | Yu Liu Xiao-Qin He Xin Huang Lu Ding Lin Xu Yu-Ting Shen Fei Zhang Mao-Bi Zhu Bai-Hui Xu Zhong-Quan Qi Hai-Long Wang |
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| Institution: | 1. Organ Transplantation Institute, Medical College, Xiamen University, Xiamen City, Fujian Province, China.; 2. Department of Gynaecology and Obstetrics, Zhongshan Hospital, Xiamen University, Xiamen City, Fujian Province, China.; 3. Center of Reproductive Medicine, Xiamen Maternity and Child Health Care Hospital, Xiamen City, Fujian Province, China.; Institute of Zoology, Chinese Academy of Sciences, China, |
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| Abstract: | Methylglyoxal, a reactive dicarbonyl compound, is mainly formed from glycolysis. Methylglyoxal can lead to the dysfunction of mitochondria, the depletion of cellular anti-oxidation enzymes and the formation of advanced glycation ends. Previous studies showed that the accumulation of methylglyoxal and advanced glycation ends can impair the oocyte maturation and reduce the oocyte quality in aged and diabetic females. In this study, we showed that resveratrol, a kind of phytoalexin found in the skin of grapes, red wine and other botanical extracts, can alleviate the adverse effects caused by methylglyoxal, such as inhibition of oocyte maturation and disruption of spindle assembly. Besides, methylglyoxal-treated oocytes displayed more DNA double strands breaks and this can also be decreased by treatment of resveratrol. Further investigation of these processes revealed that methylglyoxal may affect the oocyte quality by resulting in excessive reactive oxygen species production, aberrant mitochondrial distribution and high level lipid peroxidation, and resveratrol can block these cytotoxic changes. Collectively, our results showed that resveratrol can protect the oocytes from methylglyoxal-induced cytotoxicity and this was mainly through the correction of the abnormity of cellular reactive oxygen species metabolism. |
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