A Fast and Cost-Effective Method for Identifying a Polymorphism of Interleukin 28B Related to Hepatitis C |
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| Authors: | Camila da Silva Ferreira Rodrigo Martins Abreu Marlone Cunha da Silva Aline Siqueira Ferreira Paulo Dominguez Nasser Flair José Carrilho Suzane Kioko Ono |
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| Institution: | 1. University of São Paulo School of Medicine, Department of Gastroenterology, São Paulo, SP, Brazil.; 2. Federal University of Juiz de Fora, Department of Parasitology, Microbiology and Immunology, Institute of Biological Sciences, Juiz de Fora, Minas Gerais, Brazil.; Saint Louis University, United States of America, |
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| Abstract: | Approximately 170 million people are chronic carriers of hepatitis C virus (HCV). Patients with chronic hepatitis C are currently treated with pegylated interferon and ribavirin (PEG-IFN/RBV). A genome-wide association with PEG-IFN/RBV treatment response and a single nucleotide polymorphism (rs12979860) has been identified near the interleukin 28B gene that encodes interferon-λ-3. In this paper, we describe an innovative, fast, and low-cost multiplex polymerase chain reaction with confronting two-pair primers that detects the rs12979860 polymorphism. The assay is internally controlled and does not require the use of restriction endonucleases or special equipment. Moreover, the assay decreases costs, being about 40% cheaper than direct sequencing methods. |
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