Expression of membrane-associated C-reactive protein by human monocytes: indications for a selectin-like activity participating in adhesion |
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Authors: | Victoria Kolb-Bachofen Nicola Puchta-Teudt and Christine Egenhofer |
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Institution: | (1) Institute for Immunobiology, Medical Faculty, Heinrich-Heine-University Düsseldorf, D-40225 Düsseldorf, Germany |
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Abstract: | We have shown previously that rat liver macrophages (Kupffer cells) express a membrane-bound form of C-reactive protein (mCRP) on their surface which is identical to a galactose-specific particle receptor activity. We now establish the presence of mCRP on human monocyte-macrophages using immunocytochemistry with an anti-neoCRP specific monocloncal antibody and RNA-RNAin situ hybridization to demonstrate the presence of CRP-specific mRNA. Concomitant with mCRP expression, cells exhibit galactose-dependent uptake of particles coated with lactosylated bovine serum albumin. Adhesion experiments on fibronectin-coated surfaces that mCRP on human blood monocytes may act as a selectin-like adhesion molecule, mediating initial carbohydrate-specific contacts which are followed by peptide-specific recognition via integrin receptors. |
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Keywords: | membrane-associated C-reactive protein human monocytes adhesion molecule |
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