Chemical inhibition of sporulation of Eimeria bovis oocysts |
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| Authors: | SENGER C M |
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| Affiliation: | 1. Agricultural Experiment Station, Utah State University, ARS, U.S.D.A., Logan, Utah U.S.A.;2. Animal Disease and Parasite Research Division, ARS, U.S.D.A., Logan, Utah U.S.A.;1. Public Health Service Research Fellow of the National Institute of Allergy and Infectious Diseases, Logan, Utah U.S.A.;1. Faculdade de Ciências Agrárias e Veterinárias, UNESP/CPPAR, Via de acesso prof. Paulo Donatto Castellani, s/n CEP:14884-900, Jaboticabal, São Paulo, Brazil;2. Universidade Federal de Goiás, Regional de Jataí, Goiás, Brazil;3. UNICASTELO – Universidade Camilo Castelo Branco, Descalvado, São Paulo, Brazil;1. Escola de Veterinária e Zootecnia, Universidade Federal de Goiás. Campus Samambaia, Avenida Esperança, s/n, Campus Universitário, CEP: 74690-900, Goiânia, Goiás, Brazil;2. Biological Chemistry and Crop Protection Department, Rothamsted Research, West Common, Harpenden, Hertfordshire, AL5 2JQ, United Kingdom;3. EMBRAPA Arroz e Feijão, Rodovia GO-462, Km 12, Zona Rural, Caixa Postal: 179, CEP: 75375-000, Santo Antônio de Goiás, Goiás, Brazil;4. USDA, ARS, Knipling-Bushland U.S. Livestock Insects Research Laboratory and Veterinary Pest Genomics Center, 2700 Fredericksburg Road, Kerrville, TX, 78028, USA;5. Instituto de Patologia Tropical e Saúde Pública. Universidade Federal de Goiás, Rua 235s/n, Setor Universitário, CEP: 74605050, Goiânia, Goiás, Brazil;1. Escola de Veterinária, Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais, Brazil;2. Instituto de Farmacologia e Biologia Molecular, Universidade Federal de São Paulo, São Paulo, São Paulo, Brazil;3. Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais, Brazil;4. Escola de Veterinária e Zootecnia, Universidade Federal de Goiás, Goiânia, Goiás, Brazil;5. Centro de Ciências Biológicas, Universidade Federal de Santa Catarina, Florianópolis, Santa Catarina, Brazil;6. Centro de Ciências Agroveterinárias, Universidade do Estado de Santa Catarina, Lages, Santa Catarina, Brazil;1. São Paulo State University (Unesp), School of Veterinary Medicine and Animal Science, Botucatu, SP, 18607400, Brazil;2. Poisonous Plant Research Laboratory, Agricultural Research Service, United States Department of Agriculture, 1150 E. 1400 N., Logan, UT, 84341, USA;3. Escola de Veterinaria e Zootecnia, Universidade Federal de Goias (UFG), Goiania, GO, 74001-970, Brazil;1. Equine Sports Medicine and Surgery, 2991 West Interstate 20, South Frontage Road, Weatherford, TX 76087, USA;2. College of Veterinary Medicine, North Carolina State University, 1060 William Moore Drive, Raleigh, NC 27607, USA;3. Department of Medicine and Epidemiology, School of Veterinary Medicine, University of California, One Shields Avenue, Davis, CA 95616, USA |
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| Abstract: | Sporulation of oocysts of E. bovis was not inhibited by 0.1 M solutions of a number of salts, by oxidizing agents, by such enzyme inhibitors as fluoride, iodoacetate, ferricyanide, and arsenate, and by 1 M solutions of mineral acids and bases. Until further information is available it is logical to assume this is due to a failure of the ions to penetrate the oocyst wall.Phenol, formaldehyde, formic acid, methanol, ethanol, mercuric chloride, iodine, and ammonium hydroxide inhibited sporulation, apparently by denaturation of the proteinaceous materials. The inhibiting effect of both cyanide and azide suggests the presence of cytochrome oxidase or other cytochrome system in the sporulating oocyst. An oxidative phosphorylation system is indicated by the inhibition of sporulation by 2,4-dinitrophenol.Oocysts did not sporulate under anaerobic conditions even when such oxidizing agents as potassium dichromate and methylene blue were added. An oxygen tension of as low as 15 mm of Hg. in the atmosphere above the suspension of oocysts was sufficient for sporulation.An inhibition of sporulation in solutions saturated with carbon dioxide and carbonate ions suggests that release of CO2 must accompany sporulation. |
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