Intersectin 1 forms a complex with adaptor protein Ruk/CIN85 in vivo independently of epidermal growth factor stimulation |
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| Authors: | Oleksii Nikolaienko Inessa Skrypkina Liudmyla Tsyba Yaroslav Fedyshyn Dmytro Morderer Vladimir Buchman Susana de la Luna Lyudmyla Drobot Alla Rynditch |
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| Institution: | 1. Medical Research Center, Guangdong General Hospital, Guangdong Provincial Cardiovascular Institute, Guangdong Academy of Medical Sciences, Guangzhou, Guangdong 510080, PR China;2. Texas Heart Institute, Houston, TX 77030, United States;3. University of Texas Health Science Center, Houston, TX 77030, United States;4. Department of Urology, West China Hospital, Sichuan University, Chengdu, PR China |
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| Abstract: | Intersectin 1 (ITSN1) is an adaptor protein involved in clathrin-mediated endocytosis, apoptosis, signal transduction and cytoskeleton organization. Here, we show that ITSN1 forms a complex with adaptor protein Ruk/CIN85, implicated in downregulation of receptor tyrosine kinases. The interaction is mediated by the SH3A domain of ITSN1 and the third or fourth proline-rich blocks of Ruk/CIN85, and does not depend on epidermal growth factor stimulation, suggesting a constitutive association of ITSN1 with Ruk/CIN85. Moreover, both proteins colocalize in MCF-7 cells with their common binding partner, the ubiquitin ligase c-Cbl. The possible biological role of the interaction between ITSN1 and Ruk/CIN85 is discussed. |
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