Characterization of a bacteriocin produced by Streptococcus thermophilus ST134 |
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Authors: | D. J. Ward G. A. Somkuti |
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Affiliation: | (1) U.S. Department of Agriculture, ARS, Eastern Regional Research Center, 600 E. Mermaid Lane, 19118 Philadelphia, PA, USA |
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Abstract: | A pH-dependent adsorption/desorption technique was used to screen Streptococcus thermophilus strains for the production of bacteriocins. Agar-diffusion tests with S. thermophilus strains as targets identified 13 out of 41 strains as producers of antibacterial activity. Thermophilin A, the bacteriocin-like substance present in the culture supernatant of S.thermophilus ST134 was purified to homogeneity by ammonium sulfate precipitation and ion-exchange chromatography, followed by ultrafiltration. Thermophilin A is a relatively heat-stable and apparently glycosylated bacteriocin with a bactericidal mode of action against sensitive cells. |
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