Immunohistochemical colocalization of insulin,aromatic L-amino acid decarboxylase and dopamine beta-hydroxylase in islet B cells of chicken pancreas |
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| Authors: | Tohru Watanabe Ikuko Nagatsu |
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| Institution: | (1) Department of Veterinary Anatomy, Faculty of Agriculture, Nagoya University, Nagoya, Japan;(2) Department of Anatomy, School of Medicine, Fujita-Gakuen University, Toyoake, Aichi, Japan;(3) Department of Veterinary Anatomy, Faculty of Agriculture, Nagoya University, 464-01 Nagoya, Japan |
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| Abstract: | Summary The identity of the monoamine which produces a very weak formaldehyde-induced fluorescence in some pancreatic islet cells was studied by fluorescence microscopy and immunohistochemistry either on the same tissue section or on serial tissue sections of tissue from male chickens. Pancreatic islet cells showing this very weak formaldehyde-induced fluorescence react immunohistochemically with antisera directed against insulin, aromatic L-amino acid decarboxylase and dopamine beta-hydroxylase and therefore appear to be islet B cells producing insulin and noradrenaline. |
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| Keywords: | B cells Insulin Catecholamine synthesizing enzymes Immunohistochemistry Chicken |
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