High-performance liquid chromatographic determination of the lecithin/sphingomyelin ratio in amniotic fluid |
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| Authors: | R. L. Briand |
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| Affiliation: | Department of Pathology, Regina General Hospital, Regina, and Department of Chemistry, University of Regina, Regina, Saskatchewan S4S OA2 Canada;Department of Pathology, Reginal General Hospital, Regina, Saskatchewan S4P OW5 Canada;Department of Pathology, Regina General Hospital, Regina, and Department of Chemistry, University of Regina, Regina Saskatchewan S4S OA2 Canada |
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| Abstract: | A high-performance liquid chromatographic (HPLC) procedure has been developed for the separation of phospholipids commonly found in amniotic fluid. The chromatographic separation was achieved on a 25-cm column packed with LiChrosorb DIOL (10 μm). A 3-cm column packed with silica was fitted between the injector and the DIOL column to provide complete separation of lecithin (L) and sphingomyelin (S) from the remaining amniotic fluid phospholipids. The eluted phospholipids were quantitated employing an ultraviolet absorption detector set at 203 nm. The new HPLC separation described herein has improved the resolution and peak sharpness of L and S. Furthermore, phosphatidyl glycerol and phosphatidyl inositol were completely separated and quantitated. Amniotic fluid L/S ratios determined by this technique have been compared to those of an established thin-layer chromatographic procedure. |
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