Evaluation of biosorption potency of <Emphasis Type="Italic">Acinetobacter</Emphasis> sp. for removal of hexavalent chromium from tannery effluent |
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Authors: | Shaili Srivastava Indu Shekhar Thakur |
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Institution: | (1) School of Environmental Sciences, Jawaharlal Nehru University, New Delhi, 110 067, India |
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Abstract: | Two bacterial consortia were developed by continuous enrichment of microbial population of tannery and pulp and paper mill
effluent contained Serratia mercascens, Pseudomonas fluorescence, Escherichia coli, Pseudomonas aeruginosa and Acinetobacter sp. identified by 16S rDNA method. The consortia evaluated for removal of chromate (Cr(VI)] in shake flask culture indicated
pulp and paper mill consortium had more potential for removal of chromate. Acinetobacter sp. isolated from pulp and paper mill consortium removed higher amount of chromate Cr(VI)] under aerobic conditions. Parameters
optimized in different carbon, nitrogen sources, and pH, indicated maximum removal of chromate in sodium acetate (0.2%), sodium
nitrate (0.1%) and pH 7 by Acinetobacter sp. Bacteria was applied in 2-l bioreactor significantly removed chromate after 3 days. The results of the study indicated
removal of more than 75% chromium by Acinetobacter sp. determined by diphenylcarbazide colorimetric assay and atomic absorption spectrophotometer after 7 days. Study of microbial
Cr(VI)] removal and identification of reduction intermediates has been hindered by the lack of analytical techniques. Therefore,
removal of chromium was further substantiated by transmission electron microscopy (TEM), scanning electron microscopy (SEM)
and energy-dispersive X-ray spectroscopy (EDX) which indicated bioaccumulation of chromium in the bacterial cells. |
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Keywords: | Acinetobacter sp Biosorption Chromate Transmission electron microscopy Scanning electron microscopy Energy-dispersive X-ray spectroscopy |
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