Columnar denitrification of water by immobilized Pseudomonas denitrificans cells |
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Authors: | Inge Nilsson Sten Ohlson |
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Affiliation: | (1) Department of Technical Microbiology, Chemical Center, University of Lund, P.O. Box 740, S-22007 Lund 7, Sweden;(2) Department of Pure and Applied Biochemistry, Chemical Center, University of Lund, P.O. Box 740, S-220 07 Lund 7, Sweden;(3) Present address: Gambro AB, P.O. Box 10101, S-220 10 Lund, Sweden |
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Abstract: | Summary Whole cells of Pseudomonas denitrificans, immobilized in alginate gel, were used for columnar denitrification of ground water. Ethanol was selected as a suitable carbon source and the C/N-ratio necessary for satisfactory nitrate reduction was established (1.6 mg ethanol-C/mg nitrate-N). The course of the reaction and the diffusional limitations were investigated during columnar denitrification. The mechanical integrity of the gel matrix, as judged from leakage of cells was studied. The release of cells into the effluent was effectively inhibited (<102 cells/ml) by the use of different filter devices. The operational characteristics were determined by studying a column operating for nearly four months. Theoretically, the alginate gel column should, from high nitrate drinking water (22 mg NO3–-N/1), produce 3 1 of denitrified water/kg gel/h (wet wt.) during a period of two months. The regeneration of nitrate reduction activity by means of activation in nutrient media proved a useful tool for restoring initial activity, the gel column having shown no loss in activity at the end of the operation period. |
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