Chromosomal localization of Sau3A repetitive DNA revealed by in situ hybridization |
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| Authors: | Ryoiti Kiyama Michio Oishi Naotoshi Kanda |
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| Institution: | (1) Institute of Applied Microbiology, University of Tokyo, Bunkyo-ku, 113 Tokyo, Japan;(2) Department of Anatomy, Tokyo Women's Medical College, 8-1 Kawada-cho, Shinjuku-ku, 162 Tokyo, Japan;(3) Present address: Department of Health & Human Services, National Institute of Health, 20892 Bethesda, MD, USA |
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| Abstract: | The Sau3A DNA family consists of unique alphoid human repetitive DNA which is prone to be excised from the chromosomes and exhibits restriction fragment length polymorphism. We studied the chromosomal localization of the DNA by in situ hybridization using cultured normal human lymphocytes. Under standard hybridization conditions, the sequence hybridized with the centromeric regions of chromosomes 1, 2, 4, 11, 15, 17, 18, 19 and X, but under high stringency hybridization conditions, it hybridized with the centromeric regions of chromosomes 1, 17 and X, and particularly chromosome 11. Based on these results, we discuss the evolutionary relationship among the sequences of the Sau3A DNA family. |
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