| CRISPR在食源性致病菌进化分析、检测分型及毒力耐药调控中的应用进展 |
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| 引用本文： | 白芷烨,汪雯,吉小凤,肖英平,张世钦,王梓晨,李红梅,董庆利. CRISPR在食源性致病菌进化分析、检测分型及毒力耐药调控中的应用进展[J]. 生物工程学报, 2021, 37(7): 2414-2424 |
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| 作者姓名： | 白芷烨 汪雯 吉小凤 肖英平 张世钦 王梓晨 李红梅 董庆利 |
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| 作者单位： | 1 上海理工大学 医疗器械与食品学院，上海 200093;2 浙江省农业科学院农产品质量安全与营养研究所 农业农村部农产品质量安全风险评估实验室 (杭州) 农产品质量安全危害因子与风险防控国家重点实验室，浙江 杭州 310021 |
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| 基金项目： | 国家重点研发计划 (No. 2019YFE0103800)，浙江省农业科学院省部共建农产品质量安全国家重点实验室开放基金项目 (No. 2010DS700124-KF2001) 资助。 |
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| 摘 要： | 规律成簇间隔的短回文重复序列(Clustered regularly interspaced short palindromic repeats,CRISPR)与其相关蛋白基因系统可通过限制基因的水平转移而有效防御噬菌体等外源基因元件的入侵,不同细菌之间的CRISPR结构有所差异.基于CRISPR系统的差异性,文中对近...
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| 关 键 词： | CRISPR 食源性致病菌 进化分析 检测与分型 毒力与耐药 |
| 收稿时间： | 2020-08-25 |
Application of CRISPR in evolution analysis, detecting and typing, virulence and antibiotic resistance regulation in food-borne pathogens |
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| Zhiye Bai,Wen Wang,Xiaofeng Ji,Yingping Xiao,Shiqin Zhang,Zichen Wang,Hongmei Li,Qingli Dong. Application of CRISPR in evolution analysis, detecting and typing, virulence and antibiotic resistance regulation in food-borne pathogens[J]. Chinese journal of biotechnology, 2021, 37(7): 2414-2424 |
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| Authors: | Zhiye Bai Wen Wang Xiaofeng Ji Yingping Xiao Shiqin Zhang Zichen Wang Hongmei Li Qingli Dong |
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| Affiliation: | 1 School of Medical Instrument and Food Engineering, University of Shanghai for Science and Technology, Shanghai 200093, China;2 State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-products, MOA Laboratory of Quality & Safety Risk Assessment for Agro-products (Hangzhou), Institute of Quality and Standard of Agricultural Products, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, Zhejiang, China |
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| Abstract: | Clustered regularly interspaced short palindromic repeats (CRISPR) and its associated protein gene system can limit the horizontal gene transfer, thereby effectively preventing the invasion of foreign gene elements such as bacteriophages. CRISPR arrays of different bacteria are diverse. Based on the differences in the CRISPR system, this review summarizes the application of CRISPR in food-borne pathogen evolution analysis, detection and typing, virulence and antibiotic resistance in recent years. We also address bacterial detection typing method developed based on the characteristics of CRISPR arrays and the association of CRISPR with virulence and drug resistance of food-borne pathogens. The shortcomings of CRISPR in evolution, detection and typing, virulence and resistance applications are analyzed. In addition, we suggest standardizing CRISPR typing methods, improving and expanding the CRISPR database of pathogenic bacteria, and further exploring the co-evolution relationship between phages and bacteria, to provide references for further exploration of CRISPR functions. |
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| Keywords: | CRISPR food-borne pathogens evolutionary analysis detection and typing virulence and antibiotic resistance |
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