| 小鼠杂交瘤单克隆抗体快速制备技术研究进展 |
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| 引用本文： | 方水琴,刘程,马俊飞,闫帅帅,许东坡,刘箐. 小鼠杂交瘤单克隆抗体快速制备技术研究进展[J]. 生物工程学报, 2021, 37(7): 2293-2306 |
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| 作者姓名： | 方水琴 刘程 马俊飞 闫帅帅 许东坡 刘箐 |
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| 作者单位： | 上海理工大学 医疗器械与食品学院，上海 200093 |
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| 基金项目： | 国家重点研发计划 (Nos. 2018YFC1602900，2018YFC1602500) 资助。 |
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| 摘 要： | 小鼠杂交瘤单克隆抗体来源稳定、后期易制备、产量高,是免疫学中使用最为普遍的抗体.传统的耗时费力的杂交瘤制备技术无法满足日益增长的市场需求.文中从抗原设计筛选、B细胞富集与筛选、骨髓瘤细胞的改造、融合技术的改进、阳性杂交瘤细胞筛选及单克隆抗体性能快速测定中所涉及的快速制备技术方面进行阐述,以期为系统化的小鼠杂交瘤单克隆抗...
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| 关 键 词： | 小鼠杂交瘤 单克隆抗体 快速制备 抗原特异性 |
| 收稿时间： | 2020-05-16 |
Research progress in the rapid preparation of monoclonal antibodies of mouse hybridoma |
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| Shuiqin Fang,Cheng Liu,Junfei M,Shuaishuai Yan,Dongpo Xu,Qing Liu. Research progress in the rapid preparation of monoclonal antibodies of mouse hybridoma[J]. Chinese journal of biotechnology, 2021, 37(7): 2293-2306 |
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| Authors: | Shuiqin Fang Cheng Liu Junfei M Shuaishuai Yan Dongpo Xu Qing Liu |
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| Affiliation: | School of Medical Instrument and Food Engineering, University of Shanghai for Science and Technology, Shanghai 200093, China |
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| Abstract: | Mouse hybridoma monoclonal antibody is the most commonly used antibody in immunology because of its stable source, easy preparation in later stage and high yield. The traditional time-consuming and laborious hybridoma preparation technology could not meet the growing market demand. In this paper, we describe the rapid preparation techniques involved in antigen design and screening, B cell enrichment and screening, transgenic myeloma cells, fusion technology improvement, positive hybridoma cell screening and rapid detection of monoclonal antibody performance, to provide a reference for rapid preparation of mouse hybridoma monoclonal antibody. |
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| Keywords: | mouse hybridoma monoclonal antibody rapid preparation antigen-specific |
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