Crystallization and preliminary X-ray diffraction studies of mandelonitrile lyase from almonds |
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Authors: | Hanspeter Lauble Kirsten Müller Hermann Schindelin Siegfried Frster Franz Effenberger |
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Institution: | Hanspeter Lauble,Kirsten Müller,Hermann Schindelin,Siegfried Förster,Franz Effenberger |
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Abstract: | Single crystals of three different isoenzymes of (R)?(+) mandelonitrile lyase (hydroxynitrile lyase) from almonds (Prunus amygdalus) have been obtained by hanging drop vapor diffusion using polyethylene glycol 4000 and isopropanol as co-precipitants. The crystals belong to the monoclinic space group P2l with unit cell parameters a = 69.9, b = 95.1, c = 95.6 Å, and β = 118.5°. A complete set of diffraction data has been collected to 2.6 Å resolution on native crystals of isoenzyme III. © 1994 Wiley-Liss, Inc. |
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Keywords: | hydroxynitrile lyase flavoenzyme X-ray crystallography |
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