Simple method for clinical determination of 13 carotenoids in human plasma using an isocratic high-performance liquid chromatographic method |
| |
Authors: | Bernard Lyan, V ronique Azaï s-Braesco, Nicolas Cardinault, Viviane Tyssandier, Patrick Borel, Marie-C cile Alexandre-Gouabau,Pascal Grolier |
| |
Affiliation: | Bernard Lyan, Véronique Azaïs-Braesco, Nicolas Cardinault, Viviane Tyssandier, Patrick Borel, Marie-Cécile Alexandre-Gouabau,Pascal Grolier, |
| |
Abstract: | We report a reversed-phase high-performance liquid chromatography method which resolves 13 identified carotenoids and nine unknown carotenoids from human plasma. A Nucleosil C18 column and a Vydac C18 column in series are used with an isocratic solvent system of acetonitrile–methanol containing 50 mM acetate ammonium–dichloromethane–water (70:15:10:5, v/v/v/v) as mobile phase at a flow-rate of 2 ml/min. The intra-day (4.5–8.3%) and inter-day (1.3–12.7%) coefficients of variation are suitable for routine clinical determinations. |
| |
Keywords: | Carotenoids |
本文献已被 ScienceDirect 等数据库收录! |
|