1.Departamento de Biología de Plantas,Centro de Investigaciones Biológicas, CSIC,Madrid,Spain
Abstract:
Background
Double-stranded RNA (dsRNA) is a potent initiator of gene silencing in a diverse group of organisms that includes plants,
Caenorhabditis elegans, Drosophila and mammals. We have previously shown and patented that mechanical inoculation of in vitro-transcribed dsRNA derived from viral sequences specifically prevents virus infection in plants. The approach required the
in vitro synthesis of large amounts of RNA involving high cost and considerable labour.