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Immunological detection of cloned antigenic genes of Vibrio cholerae in Escherichia coli |
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| Authors: | Ranjana Srivastava AA Khan Brahm S Srivastava |
| Institution: | Cholera Immunology, Microbiology Division, Central Drug Research Institute, Lucknow 226001, India Tel. 32411 |
| Abstract: | Antibodies have been used as probe to detect cloned genes coding for toxin and surface antigens of Vibrio cholerae E1 Tor strain KB207. Eco RI-digested chromosomal DNA of KB207 was cloned in plasmid pBR325 and transformed in Escherichia coli HB 101(λcI857). Transformants were grown at 32° C on plates containing antibodies. Lysogen was induced at 42 °C to release expressed antigens. Antigen-antibody reaction produced a halo around positive clones. |
| Keywords: | Recombinant DNA antibody probe antigenic genes phage lysogens pBR plasmids |
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