Response of primed human PBMC to synthetic peptides derived from hepatitis B virus envelope proteins: a search for promiscuous epitopes |
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Authors: | Doh Hyounmie Roh Sujin Lee Kyung Wha Kim Kilhyoun |
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Affiliation: | Division of Molecular Life Sciences and College of Pharmacy, Ewha Womans University, 11 Daehyun-Dong, Seoul 120-750, South Korea. |
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Abstract: | This investigation was aimed at identifying effective T helper cell epitopes to the hepatitis B virus in humans. A panel of synthetic peptides that represent the hepatitis B virus whole envelope proteins was examined for their capability to stimulate peripheral blood mononuclear cells from human subjects infected with hepatitis B virus naturally. In addition, a large number of subjects were examined and their human leukocyte antigen (HLA) class II allele types were identified to determine whether the helper T cell epitope is specific for a particular HLA allele or 'promiscuous'. The peptides of the amino acid residues 52-67, 110-125, 190-205, and 228-243 appeared to be immunogenic, and particularly, the 52-67 residue was the most promiscuous epitope peptide. These results would contribute to the better understanding of the helper T cell responses to the hepatitis B virus and provide a useful way in designing epitope-based vaccines and future therapeutic strategies. |
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Keywords: | Hepatitis B virus Peripheral blood mononuclear cell T helper epitope |
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