| Impacts of Elevated CO2 Concentration on Biochemical Composition, Carbonic Anhydrase, and Nitrate Reductase Activity of Freshwater Green Algae |
| |
| 作者姓名： | Jian-RongXIA Kun-ShanGAO |
| |
| 作者单位： | [1]MarineBiologyInstitute,ShantouUniversity,Guangdong515063,China／／InstituteofHydrobiology,theChineseAcademyofSciences,Wuhan430072,China [2]MarineBiologyInstitute,ShantouUniversity,Guangdong515063,China／／InstituteofHydrobiology,theChineseAcademyofSciences,Wuhan430072,China |
| |
| 摘 要： | To investigate the biochemical response of freshwater green algae to elevated CO2 concentrations, Chlorella pyrenoidosa Chick and Chlamydomonas reinhardtii Dang cells were cultured at different CO2 concentrations within the range 3-186μmol/L and the biochemical composition, carbonic anhydrase (CA),and nitrate reductase activities of the cells were investigated. Chlorophylls (Chl), carotenoids, carbonhydrate,and protein contents were enhanced to varying extents with increasing CO2 concentration from 3-186μmol/L. The CO2 enrichment significantly increased the Chl a/Chl b ratio in ChloreUa pyrenoidosa, but not in Chlamydomonas reinhardtii. The CO2 concentration had significant effects on CA and nitrate reductase activity. Elevating CO2 concentration to 186μmol/L caused a decline in intracellular and extracellullar CA activity. Nitrate reductase activity, under either light or dark conditions, in C. reinhardtii and C. pyrenoidosa was also significantly decreased with CO2 enrichment. From this study, it can be concluded that CO2 enrichment can affect biochemical composition, CA, and nitrate reductase activity, and that the biochemical response was species dependent.
|
| 关 键 词： | 二氧化碳浓度 碳酸酐酶 硝酸盐还原酶 酶活性 绿藻 生物化学成分 Chlorella pyrenoidosa Chlamydomonas reinhardtii |
Impacts of Elevated CO2 Concentration on Biochemical Composition, Carbonic Anhydrase, and Nitrate Reductase Activity of Freshwater Green Algae |
| |
| Authors: | Jian-Rong XIA Kun-Shan GAO |
| |
| Institution: | Marine Biology Institute, Shantou University, Guangdong 515063, China;;;Institute of Hydrobiology, the Chinese Academy of Sciences, Wuhan 430072, China) |
| |
| Abstract: | Abstract: To investigate the biochemical response of freshwater green algae to elevated CO2 concentrations, Chlorella pyrenoidosa Chick and Chlamydomonas reinhardtii Dang cells were cultured at different CO2 concentrations within the range 3‐186 ümol/L and the biochemical composition, carbonic anhydrase (CA), and nitrate reductase activities of the cells were investigated. Chlorophylls (Chl), carotenoids, carbonhydrate, and protein contents were enhanced to varying extents with increasing CO2 concentration from 3‐186 ümol/L. The CO2 enrichment significantly increased the Chl a/Chl b ratio in Chlorella pyrenoidosa, but not in Chlamydomonas reinhardtii. The CO2 concentration had significant effects on CA and nitrate reductase activity. Elevating CO2 concentration to 186 ümol/L caused a decline in intracellular and extracellullar CA activity. Nitrate reductase activity, under either light or dark conditions, in C. reinhardtii and C. pyrenoidosa was also significantly decreased with CO2 enrichment. From this study, it can be concluded that CO2 enrichment can affect biochemical composition, CA, and nitrate reductase activity, and that the biochemical response was species dependent. (Managing editor: Ping HE) |
| |
| Keywords: | carbonic anhydrase Chlamydomonas reinhardtii Chlorella pyrenoidosa CO2 nitrate reductase |
| 本文献已被 维普 等数据库收录！ |
|
