A rapid method for resectioning of semithin large epoxy sections for electron microscopy

A simple and rapid method is described for resectioning semithin large epoxy tissue sections. Six-micron thick sections are cut, floated on a polystyrene coverslip, and stained with toluidine blue. The coverslip is trimmed to the size of a BEEM capsule cap and placed section face-up inside the cap. A BEEM capsule with the conical end cut off is fitted onto the cap, filled with plastic and polymerized. The capsule and coverslip are trimmed off and thin sectioning is performed. This method allows the study of multiple tissue lesions in their entirety, i.e. serially from beginning to end, without sacrificing any part during trimming as in conventional thin sectioning.