Properties of hemocyanins isolated from Amazon river arthropods and molluscs |
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| Affiliation: | 1. Department of Biochemistry, Duke University Medical Center and Duke University Marine Laboratory, Beaufort, NC 28516, U.S.A.;2. Istituto di Chimica Biologica, Centro di Biologia Molecolare, Citta Universitaria, 00185 Rome, Italy;3. Department of Chemistry, University of Essex, Wivenhoe Park, Colchester CO4 35Q, England;4. Department of Zoology, University of Texas at Austin, Austin, TX 78712, U.S.A.;5. Department of Biology, San Francisco State University, San Francisco, CA 94132, U.S.A.;1. Key Laboratory of Aquatic Product Processing, Ministry of Agriculture and Rural Affairs, National R&D Center for Aquatic Product Processing, South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou 510300, China;2. Graduate School of Marine Science and Technology, Tokyo University of Marine Science and Technology, 4-5-7 Konan, Minato, Tokyo 108-8477, Japan;3. College of Food Science and Technology, Shanghai Ocean University, Laboratory of Quality and Safety Risk Assessment for Aquatic Products on Storage and Preservation (Shanghai), Ministry of Agriculture, National R&D Branch Center for Freshwater Aquatic Products Processing Technology (Shanghai), Shanghai 201306, China;1. Guangdong Provincial Key Laboratory of Marine Biotechnology, Institute of Marine Sciences, Shantou University, Shantou, 515063, China;2. Fujian Provincial Key Laboratory of Food Microbiology and Enzyme Engineering, College of Ocean Food and Biological Engineering, Jimei University, Xiamen, 361021, China;3. Key Laboratory of Tropical Marine Bio-resources and Ecology, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou, 510301, China |
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| Abstract: | - 1. Hemocyanins from four organisms inhabiting the Amazon River were isolated and partially characterized.
- 2. Three arthropodan species (Dilocarcinus pagei cristatus, Silviocarcinus pardalinus andMacrobrachium amazonicum) possess hemocyanins whose subunit structure is remarkably simple. Regular and SDS polyacrylamide disc electrophoresis revealed predominantly single bands and no polymorphisms.
- 3. Oxygen-binding experiments showed that the three arthropodan hemocyanins possess large positive Bohr effects and pH dependence in the degree of subunit interaction.
- 4. The hemocyanin of one mollusc,Pila sp., was studied and its subunit size appears to be similar to that of other molluscan hemocyanins, i.e. a polypeptide of mol. wt 400,000. In the hemolymph,Pila hemocyanin probably exists as a mixture of 100 and 124S aggregates.
- 5. The oxygen binding properties of the large molecules ofPila hemocyanin are notable because of their low cooperativity and lack of a strong pH-dependence.
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