口服醋酸棉酚前后人精子体外受精能力及精核转变的细胞学观察

本文报道口服醋酸棉酚对人精子体外受精能力、原核及染色体形成的影响。结果表明,在口服醋酸棉酚前的9名男性精子对金黄地鼠卵的穿透率平均为62%。每日口服20mg醋酸棉酚15天后,精子对卵的穿透率下降至47%,30天后下降至24%,50天后下降至8%。即口服醋酸棉酚50天后达到了不再具有生殖能力的精子穿透率阈值(10%)以下。原核及染色体形成的观察表明,即使口服醋酸棉酚50天后,仍可见有完整原核的形成,并未见有明显的染色体畸变。综述上列结果,似乎表明口服醋酸棉酚虽可影响人精子对去除透明带金黄地鼠卵的穿透率,而进入卵内的精子仍可形成原核及染色体。因此,仅从成熟精子的生理功能而论,口服醋酸棉酚期间它似乎并不产生可察觉的致畸效应,这与醋酸棉酚对体细胞并无致畸作用的报道相一致。

OBSERVATIONS OF IN VITRO FERTILIZATION CAPACITY AND THE TRANSFORMATION OF HUMAN SPERM NUCLEI BEFORE AND AFTER ADMINISTRATION OF GOSSYPOL ACETIC ACID

By zona-free hamster ovum pene-tration assay,the in vitro fertilizationcapicities,pronucleus and chromosomeformation of human spermatozoa takenfrom 9 volunteers,who have beenshown to be fertile,were tested.Theresults showed that the average pene-tration rate was 62% before adminis-tration of gossypol acetic acid.Aftertaking gossypol acetic acid 20 mg perday for 15 days the penetration rate de-creased to 47% and became 24%30dayslater and even as lower as 8% after 50days.However,the pronucleus andchromosomes of spermatozoa could stillbe formed even if 50 days after takinggossypol acetic acid,and no obviousabnomalities of pronucleus or chro-mosomes could be found.Therefore,itseemed that gossypol acetic acid had nomutagenic or teratogenic effect onsperm if it could be capable to penetrateinto the hamster egg.