Bovine viral diarrhoea virus antigen in foetal calf serum batches and consequences of such contamination for vaccine production. |
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| Authors: | B Makoschey P T J A van Gelder V Keijsers D Goovaerts |
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| Affiliation: | 1. Virological R&D Department, Intervet International b.v., Wim de Körverstraat 35, NL-5831 AN Boxmeer, The Netherlands;2. Biological All Purpose Pilot Plant, Intervet International b.v., NL-5831 AN Boxmeer, The Netherlands;3. Research and Development, Intervet Inc, Millsboro, DE 19966-0318, USA;1. Department of Pathology, The University of Texas Medical Branch at Galveston, United States;2. The Sealy Center for Vaccine Development, The University of Texas Medical Branch at Galveston, United States;3. The Center for Biodefense and Emerging Infectious Diseases, The University of Texas Medical Branch at Galveston, United States;4. Galveston National Laboratory, The University of Texas Medical Branch at Galveston, United States;1. Department of Chemical Engineering and Polymer Research Center, Bogazici University, Bebek, Istanbul, Turkey;2. Department of Bioinformatics and Genetics, Faculty of Engineering and Natural Sciences, Kadir Has University, Cibali, Istanbul, Turkey;1. KU Leuven – University of Leuven, Department of Microbiology and Immunology, Rega Institute for Medical Research, Laboratory of Virology and Chemotherapy, B-3000, Leuven, Belgium;2. KU Leuven – University of Leuven, Department of Pharmaceutical and Pharmacological Sciences, Rega Institute for Medical Research, Medicinal Chemistry, B-3000, Leuven, Belgium;3. KU Leuven, Division Animal and Human Health Engineering, Laboratory for Host Pathogen Interactions, Kasteelpark Arenberg 30, 3001, Leuven, Belgium;4. Université Clermont Auvergne, Université d''Auvergne, IMTV, BP 10448, F-63000, Clermont-Ferrand, France;5. Inserm, UMR 990, IMTV, F-63005, Clermont-Ferrand, France;1. Department of Physiology & Biophysics, Virginia Commonwealth University, Richmond, Virginia;2. Department of Medicine, Pulmonary Section, University of Illinois at Chicago, Illinois;3. Department of Chemistry, University of Illinois at Chicago, Illinois;4. Department of Biology, University of Erlangen-Nürnberg, Erlangen, Germany;1. Emerging Infectious Diseases Branch, Walter Reed Army Institute of Research, Silver Spring, MD, USA;2. International Vaccine Institute, Seoul, Republic of Korea |
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| Abstract: | A protocol to test foetal calf serum (FCS) for contamination with bovine viral diarrhoea virus (BVDV) is described. Following this protocol, which combines cell culture methods and detection of pestivirus RNA, seven batches of FCS were tested. Infectious BVDV was detected in four of those batches. One of the remaining batches contained a relatively high number of non-infectious BVDV particles. A sample of this batch was formulated with aluminium hydroxide and aluminium phosphate as adjuvant into an experimental vaccine preparation. This product was injected twice into BVDV seronegative cattle with a 4 week interval. Blood samples taken 4 weeks after the second application were negative for BVDV specific antibodies. Our data stress that detection of BVDV RNA is not sufficient for a complete risk assessment on FCS. Discrimination between infectious and non-infectious BVDV is essential. This can only be achieved by cell culture methods. |
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