A Method for Demonstrating Prophenoloxidase after Electrophoresis |
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| Authors: | K Nellaiappan A Vinayakam |
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| Institution: |
a Department of Zoology, University of Madras, Madras, India
b Post Graduate and Research Department of Zoology, Sir Theagaraya College, Madras, India |
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| Abstract: | The demonstration of prophenoloxidase after electrophoresis is based on its activation by sodium dodecyl sulfate (SDS) or sodium oleate and staining the activated phenoloxidase with dopamine and 3-methyl-2-benzothiazolinone hydrazone hydrochloride (MBTH). A rapid method is presented for demonstrating the presence of activated phenoloxidase using polyacrylamide gel electrophoresis followed by staining in the presence of SDS or sodium oleate. |
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| Keywords: | prophenoloxidase oleate activation MBTH electrophoresis |
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