T 4 -mediated v-gene reactivation of UV-irradiated phage Ti, and its comparison with host-cell reactivation

Soaked seeds of Vicia faba were irradiated with 800 or 400 R, or two 400-R exposures, both with VAC (vacuum) but one with a concomitant treatment of CHM (cycloheximide) (I μg/ml). The chromosome aberration yields from each regimen varied with fixation time such that a unique and characteristic aberration yield for each regimen relative to the others was not obtained. Were single fixations employed one could obtain yields which would indicate no, some, or maximum repair. A single fixation would lead to an incorrect estimation of chromosome damage repair.