Induction of antigen-specific immunity by pH-sensitive carbonate apatite as a potent vaccine carrier |
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Authors: | Hebishima Takehisa Tada Seiichi Takeshima Shin-nosuke Akaike Toshihiro Ito Yoshihiro Aida Yoko |
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Institution: | aViral Infectious Diseases Unit, RIKEN Advanced Science Institute, 2-1 Hirosawa, Wako, Saitama 351-0198, Japan;bNano Medical Engineering Laboratory, RIKEN Advanced Science Institute, 2-1 Hirosawa, Wako, Saitama 351-0198, Japan;cDepartment of Biomolecular Engineering, Graduate School of Bioscience and Biotechnology, Tokyo Institute of Technology, Yokohama 226-8501, Japan |
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Abstract: | The ability of carbonate apatite (CO3Ap) to enhance antigen-specific immunity was examined in vitro and in vivo to investigate its utility as a vaccine carrier. Murine bone marrow-derived dendritic cells took up ovalbumin (OVA) containing CO3Ap more effectively than free OVA. Interestingly, mice immunized with OVA-containing CO3Ap produced OVA-specific antibodies more effectively than mice immunized with free OVA. Furthermore, immunization of C57BL/6 mice with OVA-containing CO3Ap induced the proliferation and antigen-specific production of IFN-γ by splenocytes more strongly than immunization with free OVA. Moreover, no significant differences were detected in the induction of delayed-type hypersensitivity responses, an immune reaction involving an antigen-specific, cell-mediated immune response between OVA-containing CO3Ap and OVA-containing alumina salt (Alum), suggesting that CO3Ap induced cell-mediated immune response to the same degree as Alum, which is commonly used for clinical applications. This study is the first to demonstrate the induction of antigen-specific immune responses in vivo by CO3Ap. |
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Keywords: | Carbonate apatite Vaccine carrier Dendritic cells Antibody Cellular immunity |
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