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ESR Detection of 1O2 Reveals Enhanced Redox Activity in Illuminated Cell Cultures |
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| Authors: | Ronit Lavi Michael Sinyakov Amram Samuni Smadar Shatz Harry Friedmann Asher Shainberg |
| Institution: | 1. Department of Chemistry Bar-Ilan University Ramat-Gan Israel;2. The Faculty of Life Sciences Bar-Ilan University Ramat-Gan Israel;3. Department of Molecular Biology Hadassah-Hebrew University, School of Medicine, The Hebrew University Jerusalem Israel;4. Department of Physics Bar-Ilan University 52900 Ramat-Gan Israel |
| Abstract: | Low-energy visible light (LEVL) has previously been found to modulate various processes in different biological systems. One explanation for the stimulatory effect of LEVL is light-induced reactive oxygen species formation. In the present study, both sperm and skin cells were illuminated with LEVL and were found to generate singlet oxygen (1O2). The detection of 1O2 was performed using a trapping probe, 2,2,6,6-tetramethyl-4-piperidone, coupled with electron paramagnetic resonance spectroscopy. In addition, we have shown that, together with 1O2 generation, LEVL illumination increases the reductive capacity of the cells, which explains the difficulties encountered in 1O2 detection. The potential of visible light to change the cellular redox state may explain the recently observed biostimulative effects exerted by LEVL. |
| Keywords: | Singlet oxygen Photobiostimulation Light EPR TEMP LEVL low energy visible light ROS reactive oxygen species TEMP 2 2 6 6-tetramethyl-4-piperidone EPR electron paramagnetic resonance TEMPO 2 2 6 6-tetramethyl piperidine-N-oxyl 4-O-TEMPO 4-oxo-2 2 6 6-tetramethyl-piperidine-N-oxyl 4-O-TEMPO-H 1-hydroxyl-4-oxo-2 2 6 6-tetramethylpiperidine AFR ascorbyl free radical |