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Oxidation-induced calcium-dependent dehydration of normal human red blood cells
Authors:Irina M Shcherbachenko  Irina L Lisovskaya  Vladimir P Tikhonov
Institution:1. National Scientific Centre for Hematology, Novozykovsky pr. 4a, Moscow, Russiairascher@mail.ru;3. National Scientific Centre for Hematology, Novozykovsky pr. 4a, Moscow, Russia
Abstract:Phenazine-methosulphate (PMS) is a strong oxidant that induces reactive oxygen species (ROS) formation in cells. Though it has been shown that PMS increases the red blood cell (RBC) membrane permeability to K+, the hypotheses on the mechanism of PMS-induced effects are contradictory and there are no data on volume changes induced by this oxidant. Therefore, the influence of the PMS + ascorbate oxidative system on the volume of normal human RBCs was studied. In a Ca2 + -containing medium, PMS + ascorbate caused dehydration (shrinking) of RBCs judged by: (1) changes in the density and osmotic resistance distributions of RBCs, and (2) a decrease in their low-angle scattering assessed by FACS analysis. The dehydration resulted from activation of the Gardos channels, was PMS and ascorbate concentration-dependent, was associated with broadening of the density and osmotic resistance distributions of the RBCs, and decreased in the presence of the taxifolin and rutin antioxidants. These findings contribute to a better understanding of the physiology and pathology of oxidatively-modified RBCs and may be of practical significance in estimating the antioxidant activity of various substances.
Keywords:RBCs  Phenazine-methosulphate  oxidation  Gardos channels
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