Cyanide-resistant alternative respiration is strictly correlated to intracellular peroxide levels in Acremonium Chrysogenum |
| |
| Authors: | Levente Karaffa Kálmán Váczy Erzsébet Sándor Sándor Biró Attila Szentirmai István Pócsi |
| |
| Affiliation: | 1. Department of Microbiology and Biotechnology Faculty of Sciences University of Debrecen, P.O. Box 63, H-4010, Debrecen, Hungarykaraffal@tigris.klte.hu;3. Department of Microbiology and Biotechnology Faculty of Sciences University of Debrecen, P.O. Box 63, H-4010, Debrecen, Hungary |
| |
| Abstract: | A strict correlation between the intensity of the cyanide-resistant alternative respiratory pathway and the intracellular peroxide levels in the cephalosporin C producer filamentous fungus Acremonium chrysogenum was demonstrated. Intracellular peroxide levels increased in a dose-dependent manner after addition of H2O2 to the culture media. A similar phenomenon was observed due to the specific inhibition of catalase by salicylic acid. In both cases, cyanide-resistant respiration was markedly stimulated. On the other hand, both cyanide-resistant respiration and intracellular peroxide levels were effectively suppressed by the lipid peroxyl radical scavenger DL-α-tocopherol, which breaks lipid peroxidation chains effectively. Our findings firmly supported the assumption that there is a connection between the intracellular peroxide levels and the intensity of the alternative respiratory pathway in fungi. |
| |
| Keywords: | alternative respiration Acremonium chrysogenum peroxide salicylic acid DL-α-tocopherol catalase |
|
|
