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Detection of hydrogen peroxide by lactoperoxidase-mediated dityrosine formation |
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| Authors: | Ágnes Donkó Anna Orient Pál T Szabó Gábor Németh Tibor Vántus György Kéri |
| Institution: | 1. Department of Physiology, Semmelweis University, Faculty of Medicine, Budapest, Hungary;2. Chemical Research Center, Hungarian Academy of Sciences, Budapest, Hungary;3. Vichem Chemie Ltd, Budapest, Hungary;4. Pathobiochemistry Research Group of the Hungarian Academy of Sciences, Hungary;5. Pathobiochemistry Research Group of the Hungarian Academy of Sciences, Hungary |
| Abstract: | The aim of this work was to study the dityrosine-forming activity of lactoperoxidase (LPO) and its potential application for measuring hydrogen peroxide (H2O2). It was observed that LPO was able to form dityrosine at low H2O2 concentrations. Since dityrosine concentration could be measured in a simple fluorimetric reaction, this activity of the enzyme was utilized for the measurement of H2O2 production in different systems. These experiments successfully measured the activity of NADPH oxidase 4 (Nox4) by this method. It was concluded that LPO-mediated dityrosine formation offers a simple way for H2O2 measurement. |
| Keywords: | Lactoperoxidase dityrosine NADPH oxidase 4 hydrogen peroxide reactive oxygen species |