[6]-Gingerol prevents UVB-induced ROS production and COX-2 expression in vitro and in vivo |
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| Authors: | Jin-Kyoung Kim Younghwa Kim Kwang-Min Na Young-Joon Surh |
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| Affiliation: | 1. Department of Dermato-Immunology, College of Medicine, Catholic Research Institute of Medical Science, The Catholic University of Korea, Seoul, 137-701, South Korea;2. National Research Laboratory of Molecular Carcinogenesis and Chemoprevention, College of Pharmacy, Seoul National University, Seoul, 151-742, South Korea |
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| Abstract: | [6]-Gingerol, a naturally occurring plant phenol, is one of the major components of fresh ginger (Zingiber officinale Roscoe, Zingiberaceae) and has diverse pharmacologic effects. Here, we describe its novel anti-oxidant, anti-apoptotic, and anti-inflammatory activities in vitro and in vivo. In vitro, pre-treatment with [6]-gingerol reduced UVB-induced intracellular reactive oxygen species levels, activation of caspase-3, -8, -9, and Fas expression. It also reduced UVB-induced expression and transactivation of COX-2. Translocation of NF-κB from cytosol to nucleus in HaCaT cells was inhibited by [6]-gingerol via suppression of IκBα phosphorylation (ser-32). Examination by EMSAs and immunohistochemistry showed that topical application of [6]-gingerol (30 μM) prior to UVB irradiation (5 kJ/m2) of hairless mice, also inhibited the induction of COX-2 mRNA and protein, as well as NF-κB translocation. These results suggest that [6]-gingerol could be an effective therapeutic agent providing protection against UVB-induced skin disorders. |
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| Keywords: | UVB [6]-gingerol ROS cyclooxygenase-2 NF-κB caspase |
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