Separable detection of lipophilic- and hydrophilic-phase free radicals from the ESR spectrum of nitroxyl radical in transient MCAO mice |
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| Authors: | Mayumi Yamato Takeshi Shiba Ken-Ichi Yamada Toshiaki Watanabe Hideo Utsumi |
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| Institution: | 1. Department of REDOX Medicinal Science, Faculty of Pharmaceutical Sciences;2. Innovation Center for Medical Redox Navigationyamato68@redoxnavi.med.kyushu-u.ac.jp;4. Department of Bio-function Science, Faculty of Pharmaceutical Sciences, Kyushu University, Fukuoka, Japan |
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| Abstract: | Free radicals are believed to be key factors that promote ischemia reperfusion injury in the brain. This study used the characteristic spectrum of methoxycarbonyl-PROXYL to detect free radical reactions in hydrophilic and lipophilic compartments in a transient middle cerebral artery occlusion (MCAO) mouse model. Methoxycarbonyl-PROXYL, which has a high water/octanol partition coefficient, allows the detection of nitroxyl radical in both compartments simultaneously. Free radicals generation was analysed from the enhanced ESR signal decay rate of methoxycarbonyl-PROXYL. The signal decay rate in the lipidic compartment was significantly enhanced 1 h after reperfusion following MCAO. The enhanced signal decay rate was significantly suppressed by Trolox. The accumulation of lipid peroxidation products increased by 6 h post-reperfusion and was suppressed by methoxycarbonyl-PROXYL or Trolox. These results demonstrate that information pertaining to different sites of free radical generation in vivo can be obtained simultaneously and that lipid-derived radicals are generated in transient MCAO mice. |
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| Keywords: | Nitroxide EPR ischemia reperfusion lipid peroxidation free radical |
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