Hemin-induced Erk1/2 activation and heme oxygenase-1 expression in human umbilical vein endothelial cells |
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| Authors: | Nan Chen Wei Shao Pingping Lv Shuluo Zhang Yingying Chen Li Zhu |
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| Institution: | 1. Department of Physiology, Zhejiang University School of Medicine, Hangzhou, 310000, China;2. Center of Laboratory Medicine, Huashan Hospital, Shanghai Medical College, Fudan University, Shanghai, 200040, PR China;3. Department of Gastroenterology, Putuo People's hospital of Zhoushan, Zhejiang, 316100, China |
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| Abstract: | Hemin has been reported to be protective in the pathological process, but its protective mechanisms have not been precisely defined. Hemin could induce Erk1/2 phosphorylation in astrocyte. Erk1/2 phosphorylation has been proved to be involved in many growth signals cellular transduction. However, little study has been conducted as to the relationship between hemin and Erk1/2 activation in human umbilical vein endothelial cells (HUVECs). The present study aimed to investigate the relationship between hemin and Erk1/2 phosphorylation in HUVECs. The results showed that low concentration of hemin induced and sustained phosphorylation of Erk1/2 for a long time. The HO inhibitor protoporphyrin IX zinc (II) abrogated phosphorylation of Erk1/2 induced by hemin. Biliverdin, one of the metabolites of hemin, obviously induced the Erk1/2 phosphorylation in HUVECs. Both hemin and biliverdin promoted HUVEC cell growth. The results strongly suggested that hemin could induce and sustain Erk1/2 phosphorylation in HUVECs by way of HO-1 induction and biliverdin produced from HO-1 catalysing hemin degradation. |
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| Keywords: | Endothelial cells hemin Erk |
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