Plasma protein carbonyls in nonpregnant,healthy pregnant and preeclamptic women |
| |
| Authors: | Petra LM Zusterzeel Theo PJ Mulder Wilbert HM Peters Sheila A Wiseman |
| |
| Institution: | 1. Department of Obstetrics, University Medical Centre St Radboud, Nijmegen, The Netherlands;2. Department of Gynaecology, University Medical Centre St Radboud, Nijmegen, The Netherlands;3. Unilever Research Laboratorium Vlaardingen, The Nederlands;4. Department of Gastroenterology, University Medical Centre St Radboud, Nijmegen, The Netherlands |
| |
| Abstract: | Increased reactive oxygen species (ROS) and lipid peroxidation may be implicated in the pathogenesis of preeclampsia by causing cell (membrane) damage and impaired endothelial function. Carbonyl derivatives of proteins, or protein carbonyls, may be sensitive biomarkers of ROS-mediated damage. The aim of the study was to compare levels of protein carbonyls in plasma of preeclamptic, healthy pregnant and healthy nonpregnant women.Plasma protein carbonyls were measured in 47 preeclamptic, 45 healthy pregnant and 22 healthy non-pregnant women by using a sensitive ELISA-method. ANOVA, the unpaired t-test and Pearson's correlation were used for statistical analysis.Preeclamptic women had significantly higher plasma protein carbonyl levels than healthy pregnant women (P < 0.0001). Healthy pregnant women showed significantly higher protein carbonyl levels (P < 0.001) as compared to nonpregnant controls.The higher levels of protein carbonyls as compared to nonpregnant controls suggest that increased oxygen free radical damage occurs in normal pregnancy and to a much higher extent in preeclampsia. |
| |
| Keywords: | Protein carbonyls ROS Oxidative stress Preeclampsia |
|
|
