Expression of NADPH oxidase homologues and accessory genes in human cancer cell lines,tumours and adjacent normal tissues |
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| Authors: | Agnes Juhasz Yun Ge Susan Markel Alice Chiu Linda Matsumoto Josephus van Balgooy |
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| Affiliation: | 1. Laboratory of Molecular Pharmacology, Center for Cancer Research, and Division of Cancer Treatment and Diagnosis, National Cancer Institute, NIH, , Bethesda, MD, 20892, USA;2. Department of Medical Oncology and Therapeutics Research, City of Hope Comprehensive Cancer Center, 1500 E. Duarte Road, Duarte, CA, 91010, USA |
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| Abstract: | The family of NADPH oxidase (NOX) genes produces reactive oxygen species (ROS) pivotal for both cell signalling and host defense. To investigate whether NOX and NOX accessory gene expression might be a factor common to specific human tumour types, this study measured the expression levels of NOX genes 1–5, dual oxidase 1 and 2, as well as those of NOX accessory genes NoxO1, NoxA1, p47phox, p67phox and p22phox in human cancer cell lines and in tumour and adjacent normal tissue pairs by quantitative, real-time RT-PCR. The results demonstrate tumour-specific patterns of NOX gene expression that will inform further studies of the role of NOX activity in tumour cell invasion, growth factor response and proliferative potential. |
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| Keywords: | NADPH oxidase (NOX) reactive oxygen species (ROS) human cancer human tumour cell lines hydrogen peroxide |
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