Oxidative stress induces the expression of the major histocompatibility complex in murine tumor cells |
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Authors: | Maria R Oliva Antonio Iradi Federico Garrido Mercedes Ramos Ana Maria Oltr´a Pilar Muñiz |
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Institution: | 1. Departamento de Bioquímica y Biología Molecular, Universitat de Valencia, Granada;2. Departamento de Fisiología, Facultad de Medicina, Universitat de Valencia, Granada;3. Servicio de Análisis Clínicos, Hospital Virgen de las Nieves, Granada |
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Abstract: | The effect of t-butyl hydroperoxide (t-BOOH) on the induction of the Major Histocompatibility Complex (MHC) class I genes has been studied in two cell clones (B9 and G2) of the methylcholanthrene-induced murine fibrosarcoma GR9. These two clones were selected based on their different biological and biochemical behavior specially related to their tumor induction capability when injected into a BALB/c mouse. t-BOOH (0.125mM) induced the expression of H-2 molecules in both cell clones. In B9 cell clone, in which MHC basal expression is very low or absent, t-BOOH significantly induced H-2Kd, H-2Dd and H-2Ld molecules. In G2 cell clone the expression of MHC class I genes was also enhanced by the xenobiotic, the effect being especially significant on the H-2Ld molecule which is not expressed under basal conditions. H-2 molecules expression was accompanied by the activation of the transactivator factor NFκB. These results suggest that oxidative stress may modulate the antigen expression of tumor cells and thus the immune response of the host organism.Basal levels of oxidative parameters, such as anti-oxidant enzymes, malondialdehyde (MDA) and the DNA damaged base 8-hydroxy-2′-deoxyguanosine (8-OHdG), showed differences between the two fibrosarcoma cell clones. |
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Keywords: | MHC class I antigens fibrosarcoma 8-OHdG oxidative stress NFκB |
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