Effect of N-chloroamino acids on the erythrocyte |
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Authors: | A Robaszkiewicz G Bartosz M Soszyński |
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Institution: | 1. Department of Molecular Biophysics, University of ?ód?, Banacha 12/16, 90-237 ?ód?, Polandagnieszka.robaszkiewicz@gmail.com;3. Department of Molecular Biophysics, University of ?ód?, Banacha 12/16, 90-237 ?ód?, Poland;4. Department of Biochemistry and Cell Biology, University of Rzeszów, Cegielniana 12, 35-959 Rzeszów, Poland |
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Abstract: | Amino acids present in blood plasma may be targets for oxidation and chlorination by HOCl/OCl?. N-Chloroamino acids have been reported to be less reactive, but more selective than HOCl/OCl? in their reactions; therefore, they may act as secondary mediators of HOCl/OCl?-induced injury. This study compared the effects of five N-chloroamino acids (AlaCl, LysCl, SerCl, AspCl and PheCl) on erythrocytes with the action of HOCl/OCl?. The N-chloroamino acids differed in stability and reactivity. They had a weaker haemolytic action than HOCl/OCl?; HOCl/OCl?, AlaCl and PheCl increased osmotic fragility of erythrocytes at a concentration of 1 mm. Oxidation of glutathione, formation of protein-glutathione mixed disulphides and efflux of GSSG from erythrocytes were observed for erythrocytes treated with all the employed chloroderivatives, while increased oxidation of 2′,7′-dichlorofluorescin was detected only after treatment of the cells with 1 mm HOCl/OCl?, AlaCl and PheCl. Generally, the reactivity of at least some N-chloroamino acids may be not much lower with respect to HOCl/OCl?. |
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Keywords: | Hypochlorous acid chloramines amino acids erythrocyte glutathione oxidative stress |
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