The effect of prior exercise on ex vivo induction of heme oxygenase-1 in human lymphocytes |
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Authors: | Daniella Markovitch Rex M Tyrrell |
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Institution: | 1. Sport &2. Exercise Science, School for Health, Bath, BA2 7AY, UK;3. Department of Pharmacy &4. Pharmacology, University of Bath, Bath, BA2 7AY, UK |
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Abstract: | It was postulated that prior demanding exercise would suppress the induction of the oxidant-responsive protein Heme Oxygenase-1 (HO-1) in mononuclear cells following subsequent ex vivo H2O2 treatment. Eight male subjects completed two trials in a randomized order (one rest and one exercise) and ex vivo HO-1 protein induction was determined following H2O2 treatment in lymphocytes and monocytes before and after each trial using a newly developed and reproducible assay. Lymphocytes obtained 2 h post-exercise showed a modest reduction in HO-1 protein expression in response to ex vivo treatment with H2O2 (p<0.05). The plasma concentration of the HO-1 suppressor α1-antitrypsin increased immediately post-exercise (p<0.05) and it is tentatively suggested that this may explain the modest transient reduction in ex vivo HO-1 protein induction in lymphocytes in response to an independent oxidant challenge following a prior bout of demanding exercise. |
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Keywords: | Free radicals oxidative stress hydrogen peroxide physical activity mononuclear cells inflammation |
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