A simple enzymic method for the synthesis of adenosine 5'-[alpha-32P]triphosphate on a preparative scale. |
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| Authors: | B R Martin and H P Voorheis |
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| Abstract: | A simple, rapid and inexpensive method is described for the enzymic synthesis of [alpha-32P]ATP from [32P]Pi on a preparative scale with an overall yield of 53%. The final product contained all of the detectable radioactivity (less than 99.9%) in the alpha position and has been shown to behave identically with commerically availabe [alpha-32P]ATP during the synthesis of 3':5'-cyclic AMP in the reaction catalysed by adenylate cyclase. |
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