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Fluctuation of Endogenous Levels of Free and Conjugated Gibberellins throughout Seed Maturation in Leucaena leucocephala (Lmk) De Wit |
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| Authors: | Arigayo, Sayuti Fujisawa, Sachiko Sakurai, Akira Kamiya, Yuji Adisewojo, Sasongko S. Takahashi, Nobutaka |
| Affiliation: | 1Department of Chemistry, Faculty of Mathematics and Natural Sciences, Padjadjaran University Jalan Singaperbangsa 1, Bandung, Indonesia 2The Institute of Physical and Chemical Research Wako-shi, Saitama 351-01, Japan 3Department of Agricultural Chemistry, The University of Tokyo Bunkyo-ku, Tokyo 113, Japan |
| Abstract: | Combined gas chromatography-selected ion monitoring (GC-SIM)analysis of a purified extract from seeds of Leucaena leucocephalashowed the presence of GA1, GA8, GA17, GA19, GA20, GA23, GA29and GA53. GA1, GA8 and GA29 were also found both as glucosylester-like and glucosyl ether-like conjugates, and GA20 as aglucosyl ester-like conjugate; these conjugates were analyzedas free GAs after enzyme hydrolysis. GA23 was shown to be themain free gibberellin in immature seeds (268 ng/seed), thoughits level drastically decreased during their maturation. GA1was the main free C19-gibbercllin and GA1 glucosyl ester-likeand glucosyl ether-like conjugates were found at the highestlevels in the seeds prior to maturation. Fluctuation of endogenouslevels of gibberellins is discussed in terms of seed development. (Received May 9, 1984; Accepted August 25, 1984) |
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