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In vitro shoot proliferation and production of sets from garlic and shallot |
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| Authors: | Yasseen Mohamed-Yasseen Walter E Splittstoesser Richard E Litz |
| Institution: | (1) Horticulture Department, University of Illinois, W-503 Turner Hall, 1102 S. Goodwin Ave., 61801 Urbana, IL;(2) IFAS, Tropical Research & Education Center, University of Florida, 18905 SW 280 St., 33031 Homestead, FL |
| Abstract: | A procedure is described to regenerate shoots and bulbs in vitro with high frequency from shoot tips of garlic and shallot plants using benzyladenine or thidiazuron. Regenerated shoots were induced to form bulbs in Murashige and Skoog medium (1962) containing 5 g l-1 activated charcoal and 120 g l-1 sucrose under a long-day photoperiod. Bulbs formed in vitro were transferred to soil without acclimatization and produced viable plants. This method could be useful to produce low-cost bulbs, which are easy to handle and store until needed.Abbreviations AC activated charcoal - BA benzyladenine - IBA indolebutyric acid - MS Murashige & Skoog's (1962) medium - NAA naphthaleneacetic acid - TDZ thidiazuron |
| Keywords: | Allium cepa var ascalonicum Allium sativum bulb induction tissue eulture |
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