The genetic and molecular characterization of pollen-derived plant lines from octoploid triticale x wheat hybrids |
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| Authors: | Y. B. Wang H. Hu J. W. Snape |
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| Affiliation: | (1) Institute of Genetics, Chinese Academy of Sciences, 100101 Beijing, China;(2) John Innes Centre, Colney Lane, NR4 7UH Norwich, UK;(3) Present address: The Norman Borlaug Institute for Plant Science Research, De Montfort University, LE7 9SU Scraptoft, Leicester, UK |
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| Abstract: | Six doubled-haploid (DH) lines, derived by anther culture from octoploid triticale x wheat hybrids, were characterized using cytological, biochemical and molecular techniques. Lines varied in their wheat and rye genome composition, and were either wheat-rye chromosome multiple addition lines or had spontaneous substitutions and/or wheat-rye translocations. Most of the lines contained a pair of 4R chromosomes, whereas 1R or 7R were present in others. The results are similar to those previously obtained with hexaploid triticale x wheat crosses and indicate that it is possible to produce alien (wheat/rye) addition, substitution, and translocation lines directly from the anther culture of intergeneric hybrids. |
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| Keywords: | Doubled haploids Octoploid triticale Wheat Wheat/rye translocations Wheat/rye addition Genomic in situ hybridization (GISH) SDS-PAGE RFLP |
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