槐种子发育过程中子叶细胞显微结构的变化和过氧化物酶同工酶变化的动态

槐（SophorajaponicaL．）种子发育过程中，子叶细胞大量合成和积累储藏物质，光镜和电镜下用组织化学方法观察到开花后30d，叶肉细胞高度液泡化，其中分布着大量的质体、粗面内质网、一些线粒体和小脂体。淀粉粒开始积累。开花后60d，淀粉粒和脂体不断长大，数量明显增多，液泡中蛋白体开始形成。从花后90d开始，液泡中有大量蛋白体形成且不断长大，有些蛋白体变得不规则形。至种子成熟期，叶肉细胞几乎完全被蛋白体及脂体所填充。淀粉粒及大多数细胞器消失。基于上述观察，还对种子发育过程中子叶细胞过氧化物酶活力及同工酶港的变化进行了观察和分析。

MICROSTRUCTURAL CHANGES AND ISOPEROXIDASE CHANGES OF COTYLEDON CELL IN SOPHORA JAPONICA L. DURING SEED DEVELOPMENT

Accumulation and synthesis of reserve metabolites occurring in cotyledon cells dur-ing seed development were examined histochemically in Sophora japonica L.The obser-vations by light and electron microscopy show that 30 days after flowering the cotyledon mesophyll cells generally consist of highly vacuolated cells in which numerous plastids,massive rough endoplasmic reticulum. Some mitochondrion and small lipid bodies were observed and accumulation of starch grains proceeds.At 60 DAF,starch grains and lipidbodies continued to increase both in number and in size, minute protein bodies areformed along the tonoplasts. From 90 DAF,a lot of protein bodies formed in the vac-uoles and continued to increase in size. Some of them became irregular in shape. At seedmaturity,mesophyllous cell were almost filled with proteinaceous material,starch grainsand most cell organelles had disappeared. Based on above observations,Peroxidase activi-ty and the number of isoperoxidase bands of cotyledon cells have also been made duringseed development.