Genetic conversion of G.C base-pairs to A.U base-pairs in a transfer RNA |
| |
| Authors: | W H McClain J G Seidman |
| |
| Institution: | Department of Bacteriology, University of Wisconsin, Madison 53706. |
| |
| Abstract: | The cloverleaf stem segments of the suppressor gene of bacteriophage T4 tRNA(Gln) contain ten G.C and ten A.U base-pairs. To gain a better appreciation of the G.C base-pair requirement, we isolated multiple mutants of this suppressor gene in which base-pairs of G.C were replaced by A.U. One active suppressor gene contained only A.U base-pairs on the anticodon stem, indicating that G.C base-pairs in this region of tRNA(Gln) are not essential for function. In contrast, replacement was not possible at two base-pairs on the D stem and at one base-pair on the T stem. |
| |
| Keywords: | |
|
|
