| 转人α-乳清白蛋白基因烟草中半胱氨酸含量的提高 |
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| 引用本文： | 王逸群. 转人α-乳清白蛋白基因烟草中半胱氨酸含量的提高[J]. 云南植物研究, 2008, 30(4) |
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| 作者姓名： | 王逸群 |
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| 作者单位： | 福建师范大学牛命科学学院发育与神经生物学重点实验室,福建福州,350108 |
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| 基金项目： | the scientific and technological program of Fujian Science and Technology Department，福建省自然科学基金，the scientific and technological program of undergraduate students in Fujian Normal university |
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| 摘 要： | 将人α-乳清白蛋白基因构建到植物表达载体上,通过农杆菌介导采用叶盘法将人α-乳清白蛋白基因导入到烟草中,经过PCR和Southern blot分析表明:人α-乳清白蛋白基凶已经整合到烟草基因组中;经过RT-PCR葙GUS组织化学染色证明:人α-乳清白蛋白基因得到了表达.测定了9株转基因烟草叶片半胱氨酸含量,大部分植株有着明显的提高,最高幅度达到了318.02%,半胱氨酸含量平均提高了166.40%.
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| 关 键 词： | 人α-乳清白蛋白基因 转基因烟草 半胱氨酸 提高 |
Transformation of Human Alpha-lactalbumin Gene into Tobacco and Improvement of Cysteine Contents in Transgenic Tobacco Plants |
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| WANG Yi-Qun. Transformation of Human Alpha-lactalbumin Gene into Tobacco and Improvement of Cysteine Contents in Transgenic Tobacco Plants[J]. Acta Botanica Yunnanica, 2008, 30(4) |
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| Authors: | WANG Yi-Qun |
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| Abstract: | The plant expression vector containing human alpha-lactalbumin gene was constructed and its gene was transformed into tobacco using leaf disc mediated by Agrobacterium tumefac/ens LBA 4404 as a vehicle. PCR and Southern blot analyses showed that human alpha-lactalbumin gene was integrated into the tobacco genome. RT-PCR and histochemical staining for GUS activity demonstrated that human alpha-lactalbumin gene was expressed in transgenie tobacco plants. Further analysis of the data indicated that the average of eysteine contents in 9 transgenic tobacco plants was greatly increased by 166.40% besides one of them which was inereased by 318.02%. |
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| Keywords: | Human alpha-lactalbumin gene Transgenic tobacco plants Cysteine Improvement |
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