Multigene families of Cellulomonas flavigena encoding endo-beta-1,4-glucanases (CM-cellulases) |
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Authors: | M W Akhtar M Duffy B C Dowds M C Sheehan D J McConnell |
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Institution: | Department of Genetics, Trinity College, Dublin, Ireland. |
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Abstract: | Multiple genes coding for endo-beta-1,4-glucanases (CM-cellulases) have been isolated from a newly discovered highly cellulolytic strain of Cellulomonas flavigena. Clones of C. flavigena DNA were isolated in Escherichia coli and screened for gene expression on CM-cellulose plates staining with congo red. Six clones produced CM-cellulase activity as detected in liquid assays, and on activity gels. They fell into three groups within which the sequences cross-hybridised. There were small differences in the pH and temperature optima of the enzymes encoded by representatives of the three groups of clones. |
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