A simple method for measuring the kinetics of the formation of oxygen radicals |
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| Authors: | Y. Mengyan Z. Yunyun L. Qingzhi D. Yuhuan |
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| Affiliation: | (1) School of Chemical Engineering, University of Bath, Bath, UK, BA2 7AY;(2) Shijiazhuang 2nd College of High Education, Shijiazhuang, PR China;(3) Department of Chemistry, Bejing Normal University, Beijing, PR China;(4) Tangshan College of High Education, Tangshan, PR China |
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| Abstract: | Sodium 2, 6-dichloroindophenol (DCIP Na ) was used to measure the kinetics of the formation of oxygen radicals. The oxygen radicals react with DCIP - , resulting in a decrease in DCIP concentration which is monitored by the decrease in A . A method based on this principle was demonstrated with xanthine/xanthine oxidase giving a K = 4.9x10 M. Cu significantly inhibited the enzyme reaction. |
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